Spider-Man: Across the Spider-Verse premiered on May 30, 2023, at the Grand Rex in Paris, France, and at the Regency Village Theater in Los Angeles, and was theatrically released in the United States on June 2, in premium large formats and IMAX. Unlike the first film, this film was not released in 3D.

Miles Morales catapults across the multiverse, where he encounters a team of Spider-People charged with protecting its very existence.

In the storyline, Peter Parker expands the scope of his activities globally, while the now-16-year-old Miles continues to patrol New York City, and deals with issues such as confrontations with Parker's rogues gallery, the public's reaction to his ethnicity, and his love life.

Peter Parker between 30–32 years old in comics published in 2022. This is because of Marvel's sliding timescale, whereby a year in-universe is the equivalent of 4 years in the real world.